E-LASTO
Modulation of the enzymatic activity of glycoside hydrolases immobilised on elastomeric surfaces through mechanical stretching
Context
For the past three years, LCPO and TBI have been collaborating to understand and replicate the natural action of certain bacteria, particularly effective in deconstructing plant cell walls through polysaccharide hydrolysis. By controllably grafting enzymes onto a mechanically modifiable surface, it is possible to simulate the natural organisation of enzyme complexes with the aim of mastering their effect on plant cells and, more broadly, the composition of hydrolysis products.
Objectives
This project aims to develop a generic approach for the controlled and oriented immobilisation of enzymes on functionalised elastomeric surfaces.
Expected results
The technology must enable universal enzyme grafting onto a support and utilise the mechanical properties of the support as a means to modulate enzymatic reactions, thereby influencing the profile of released products.
Target markets
- Enzymes in white biotechnology processes
- Upgrading polymers from plants
Type of transfert planned
Scale-up with CRITT, demonstrators on the implementation of immobilised surfaces and research collaborations with private-sector partners:
- industries employing processes involving immobilised enzymes
Involved components
- UMR 792 TBI
- UMR 5629 LCPO